To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and ...

This map of 2,268 Drosophila melanogaster protein interactions is annotated to show nuclear, cytoplasmic, and membrane and extracellular localization. (from L. Giot et al., Science, 302:1727–36, 2003.

No protein is an island. They're linked by complex networks, and many cellular processes – transcription, translation, mitosis, and motility, to name but a few – are the work of complex macromolecular ...

Several attempts have been made to systematically map protein-protein interaction, or 'interactome', networks. However, it remains difficult to assess the quality and coverage of existing data sets.

Fluorescence resonance energy transfer (FRET) is a technique wherein two light-sensitive molecules transfer energy from a donor molecule to an acceptor molecule. The result is emittance of a ...